NO 2 , ONOO , or the ONOO generator, 3-morpholinosydnonimine (SIN-1)

Download scientific diagram | NO 2 , ONOO , or the ONOO generator, 3-morpholinosydnonimine (SIN-1) selectively kill log phase lung epithelial cells. (A ) A line of rat alveolar Type II epithelial cells (21) was used and grown to log phase or confluence and exposed to 5 ppm of NO 2 for 4 hours, and cell death was assessed immediately or 4, 24, 48 or 72 hours after cessation of exposure. (B ) Log phase or confluent rat alveolar Type II epithelial cells cells were exposed to 1 mM ONOO or 1 mM SIN-1 and death was assessed after 2, 4, and 24 hours. Cell death was assessed using an antibody recognizing single-stranded DNA (17), and results are expressed at the percentage of APOPTAG positive cells. Adapted with permission from Reference 17. *Significantly different from air controls (p 0.05). from publication: Reactive Nitrogen Species and Cell Signaling: Implications for Death or Survival of Lung Epithelium | Reactive nitrogen species such as nitric oxide, peroxynitrite, and nitrogen dioxide have been implicated in the pathophysiology of inflammatory lung diseases. Yet, the molecular mechanisms and cell signaling events responsible for cellular injury remain to be elucidated. Two | Reactive Nitrogen Species, Epithelium and Mitogen-Activated Protein Kinase Kinases | ResearchGate, the professional network for scientists.

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